Recently, our group engineered Pichia pastoris for bioproduction of 3‐hydroxypropionic acid (3-HP) from sole methanol.

3-HP is a versatile platform chemical for the synthesis of various biodegradable polymeric materials. In this study, we optimized the expression of malonyl-CoA reductase gene MCR. Then, we metabolically transformed a free fatty acid (FFA) overproducing strain toward 3-HP biosynthesis. Finally, downregulating of methanol dissimilative pathway further improved 3-HP production to 48.2 g/L with a yield of 0.23 g/g under fed-batch fermentation in bioreactor. This is the highest 3-HP production from one-carbon (C1) feedstocks reported so far.

This study was published in ACS Sustainable Chemistry & Engineering, which was supported by the National Key R&D Program of China and Dalian Science and Technology Innovation Funding. (Text and image by Xiaoyan Wu)

Link：https://pubs.acs.org/doi/10.1021/acssuschemeng.3c00410

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